| Group | Club/Team Name | Team ID | Head Coach |
| A1 | LASO Spring Companion Animal Hospital |
0114-03CM90-000056 | n/a |
| A2 | LASO Spring FC Internacional |
0114-03CM90-000058 | n/a |
| A3 | LASO Spring High Tower |
0114-03CM90-000061 | n/a |
| A4 | LASO Spring Lakeland City Players |
0114-03CM90-000060 | n/a |
| A5 | LASO Spring Lakeland United |
0114-03CM90-000057 | n/a |
| A6 | LASO Spring ML City |
0114-03CM90-000053 | n/a |
| A7 | LASO Spring Plant City FC |
0114-03CM90-000062 | n/a |
| A8 | LASO Spring Red Hoagland Hyundai |
0114-03CM90-000054 | n/a |
| A9 | LASO Spring Terror Fit |
0114-03CM90-000055 | n/a |
| A10 | LASO Spring Union FC |
0114-03CM90-000059 | n/a |